AmpFlSTR Identifiler Plus User Guide 7 Preface Purpose The Applied Biosystems AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide provides information about the Applied Biosystems instruments, chemistries, and software associated with the AmpFlSTR Identifiler Plus PCR Amplification Kit. Unless stated otherwise, the protocols in the Identifiler Plus Kit User Guide were followed (15). AmpFlSTR Identifiler PCR Amplification Kitfor use with: 200 reaction kit (Part no. Quality Assurance Manual, the accreditation standards under ISO/IEC 17025:2005, and the supplemental standards set by the FSL's accrediting body, as well as the . USER GUIDE For Research, Forensic, or Paternity Use Only. The AmpFSTR() Identifiler() Plus PCR Amplification Kit is an improved version of the AmpFSTR() Identifiler() PCR Amplification Kit and amplifies the core CODIS loci: D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA. Wear appropriate protective eyewear, clothing, and gloves. The AmpFLSTR Identifiler PCR Amplification Kit is the single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems. 4440211). no. Share to Reddit. USER GUIDE. Share to Twitter. example, the Identifiler Plus, NGM SElect or the GlobalFiler PCR Amplification Kits. AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub Item Preview remove-circle Share or Embed This Item. By adding an additional dye, more loci can be The y-axis is scaled to 130 RFU, and the artifacts are 111 Product Overview. DISC 10 AmpFlSTR Identifiler Plus User Guide Chapter 1 Overview Product overview Purpose The AmpFlSTR Identifiler Plus PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that amplifies 15 tetranucleotide repeat loci and the Amelogenin gender-determining marker in a single PCR amplification: Information in this document is subject to change without notice. Post-PCR purification decreases the remaining components left over from amplification, such as deoxyribonucleotide triphosphates, primers, salts, and enzymes so there is less competition during the electrokinetic injection and more DNA will go into the capillary. Performance Characteristics Before using this product, read and understand the information the "Safety" appendix in this document. CLA Identifiler Plus and Identifiler Direct PCR Amplification Kits USER GUIDE for Cell Line Authentication for use with: SeqStudio Genetic Analyzer 3500/3500xL Genetic Analyzer 3730/3730xl DNA Analyzer (48-capillary) 3130/3130xl Genetic Analyzer for use with: Microsatellite Analysis Software GeneMapper Software Catalog Numbers . no. USER GUIDE Catalog Numbers 4476135 (200 reactions), 4482815 (1,000 reactions) Publication Number 4477604 Revision E. The information in this guide is subject to change without notice. 1 manual. Transcription . 4440211). In addition, the Identifiler Plus Master Mix includes AmpliTaq Gold DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and . Manufacturer: Thermo Fisher Scientific | 7 Kingsland Grange | Warrington, Cheshire WA1 4SR | United Kingdom The information in this guide is subject to change without notice. The Identifiler kit is unable to amplify a blood sample from a leather glove. Identifiler Plus electropherogram generated from the DNA extraction, amplification and 3500xL fragment analysis of store purchased wheat germ. Share to Facebook. Here, we review the key performance measures of three new STR amplification systems with 6-dye chemistry, namely, the Investigator 24plex QS Kit from QIAGEN, the GlobalFiler PCR Amplification Kit from Applied Biosystems, and the PowerPlex Fusion 6C System from Promega. Purpose The Applied Biosystems AmpFlSTR Identifiler PCR Amplification Kit User Guide no. and the Identifiler Plus kit including SNP-specific primers for the vWA, D16S539, AMEL, D2S441, D22S1045, and D8S1179 loci. With its enhanced formulation, the Identifiler Plus kit helps deliver a full profile. The combination of the 15 loci are consistent with several worldwide database recommendations. . AmpFSTR Identifiler Direct PCR Amplification Kit USER GUIDE for use with: 200 reaction kit 1000 reaction kit Catalog Numbers 4467831 and 4408580 Publication Number 4415125 Revision K. Manufacturer: Thermo Fisher Scientific | 7 Kingsland Grange | Warrington, Cheshire WA1 4SR | United Kingdom The improvements in the Identifiler Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler kit. B May 2001 Update GeneAmp System 9700 part numbers. The PCR amplification reac-tion mixture was prepared in a volume of 25 lL containing 10 lLofAmpF'STR Identifiler Plus Master Mix, 5 lLof AmpF'STR Identifiler Plus Primer Set, and a maximum vol-ume of 10 lL of target DNA. . The AmpFSTR() Identifiler() Plus PCR Amplification Kit is an improved version of the AmpFSTR() Identifiler() PCR Amplification Kit and amplifies the core CODIS loci: D3S1358, D5S818 . The Applied Biosystems AmpFlSTR Identifiler PCR Amplification Kit User Guide provides information about the Applied Biosystems instruments, chemistries, and software associated with the AmpF lSTR Identifiler PCR Amplification Kit. study (sensitivity_3) was performed to evaluate the Identifiler Plus kit under the current laboratory conditions. Optimized for casework, the AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. Table 1 AmpFlSTR Identifiler Plus PCR Amplification Kit (1000 Reactions) (Part no. Table 1 AmpFlSTR Identifiler Plus PCR Amplification Kits Note: For safety and biohazard guidelines, refer to the "Safety" section in the AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub. Share to Pinterest. For both kits, 1 ng of input DNA and 28 cycles of amplification were used. Five-Dye DNA Fragment Analysis The Identifiler kit uses a five-dye fluorescent system for automated DNA fragment analysis. The recommended target DNA input for amplification using the Identifiler Plus kit at 28 cycles is approximately 1.0 ng. FBS21 - Identifiler Plus Interpretation Guidelines Page 1 of 34 Document Control Number: 2519 Approved By: Jennifer Zeffer Revision: 2 Issue Date: 2/12/2015 3:44:14 PM . 10 AmpFlSTR Identifiler Plus User Guide Chapter 1 Overview Product overview Purpose The AmpFlSTR Identifiler Plus PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that amplifies 15 tetranucleotide repeat loci and the Amelogenin gender-determining marker in a single PCR amplification: Additional loci amplified in the multiplex reaction are the sex . For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions. The 1.0 ng target produced normalized peak height from 650 to 2100 rfu. (STR kits are For Forensic or Paternity Use Only.) Quantifiler HP DNA Quantification Kit contains all the necessary reagents for the amplification, detection and quantification of two huma n-specific DNA targets. AmpFlSTR Identifiler PCR Amplification Kit User Guide 9 About This Guide IMPORTANT! The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards. 4322288) Publication Number 4323291 Rev. . Documents. The primers used in the kits are: For all loci except AMELThe same primer sequences as the NGM SElect kit and the Identifiler Plus kit including SNP-specific primers for the vWA, 4486461) Note: For safety and biohazard guidelines, refer to the "Safety" section in the AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub. These improvements enhance the assay signal-to-noise ratio and simplify the interpretation of results. Share via email. The two peaks at 176.82 bp in D8S1179 and 156.05 bp in vWA are consistent with the ID_D8-1 and ID_vWA-2 artifacts described in this document. 4486461) Note: For safety and biohazard guidelines, refer to the "Safety" section in the AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub. Helps overcome PCR inhibition through the use of a high performance PCR buffer system and optimized thermal . The new kit includes the same primers and allelic ladder as the widely used Identifil-er Kit and harnesses next-generation PCR . 4387746 Publication Number 4391294 Revision E Our results have demonstrated that GlobalFiler displays the highest . For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions. Catalog number: 4322288. Pull-out chapters This guide is designed to allow users to pull out chapters 2, 3 . Not for diagnostic procedures. Note: For safety and biohazard guidelines, refer to the "Safety" section in the Identifiler Plus PCR Amplification Kit User Guide (Part no. Quantifiler Duo DNA Quantification Kit For use with: Catalog no. 4440211). Identifiler Plus kits. AmpFlSTR Identifiler Plus PCR Amplification Kit User`s Guide Share to Tumblr. 4440211). ERR#1200 - This item cannot be ordered online. For every chemical, read the Safety Data Sheets (SDSs) and follow the handling . Wear When post-PCR purification is used; PH's will increase, PHRs should remain . For Forensic or Paternity Use Only. For every chemical, read the Safety Data Sheets (SDSs) and follow the handling . Revision Date Description A May 2001 New document. Table 1 AmpFlSTR Identifiler Plus PCR Amplification Kit (1000 Reactions) (Part no. For Forensic or Paternity Use Only. This is the User Guide for the AmpFLSTR Identifiler Plus PCR Amplification Kit. JRevision Date August 2012. Save to list. loci are consistent with the AmpFlSTR SGM Plus PCR Amplification Kit. Price (USD) 4440211.
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